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Abstract #23643 Published in IGR 11-2
Inhibitory effect of various biopolymer materials on scar formation following trabecula resection
Sha Q; Qiao X-Z; Nie Q-Z; Gao D-WJournal of Clinical Rehabilitative Tissue Engineering Research 2009; 13: 1513-1516
BACKGROUND: Fibration and scar formation are major factors for surgical failure following glaucoma filtration. Although there are so many drugs and methods to inhibit scar formation, they are not ideal. OBJECTIVE: To investigate the inhibitory effects of amniotic membrane, polylactic acid membrane, and chitosan membrane on scar formation following glaucoma filtration surgery so as to choose an ideal biopolymer material in clinical application. DESIGN, TIME AND SETTING: A randomized controlled study was performed at Central Laboratory of China Medical University between February 2007 and April 2008. MATERIALS: Amniotic membrane was provided by Ruiji Bioengineering Co., Ltd., Jiangxi; polylactic acid membrane and chitosan membrane were provided by Chinese Academy of Science. Methods: A total of 24 New Zealand white rabbits were randomly divided into 4 groups: amniotic membrane group, polylactic acid membrane group, chitosan membrane group, and control group, with 6 rabbits in each group. The left eyes underwent routine trabecula resection, and the right eyes were considered as the controls. Amniotic membrane, polylactic acid membrane, and chitosan membrane were respectively installed under sclera flap in the three groups, but any treatment was applied in the control group. MAIN OUTCOME MEASURES: Intraocular pressure, conjunctival filtering bleb, and anterior chamber inflammation response were monitored at day 1, 3, 7, 14, 28, and 56 post-operatively; eyeball tissue underwent histopathological examination at day 56 post-operatively. RESULTS: Qualitative analysis: fibrocytes and inflammatory cells were reduced in the amniotic membrane group, polylactic acid membrane group, and chitosan membrane group compared to control group. Quantitative analysis: At day 1 post-operatively, intraocular pressure was decreased in the three membrane groups compared to control group; at day 14 post-operatively, the pressure was decreased significantly, while the intraocular pressure in the three membrane groups was significantly lower than the pressure pre-operatively (P < 0.01); there were no significant differences among the three membrane groups (P > 0.05). Filtering bleb of the four groups was clearly observed at day 7 post-operatively, but there was no significant difference in pair-wise comparison. At day 28 and 56 post-operatively, filtering bleb in the control group was significantly narrowed compared to the three membrane groups (P < 0.05), but there was no significant difference in pair-wise comparison of the three membrane groups. CONCLUSION: All amniotic membrane, polylactic acid membrane, and chitosan membrane can effectively inhibit scar formation following glaucoma filtration surgery; in particular, the effect of amniotic membrane is the best. LA: Chinese
D.-W. Gao. Department of Ophthalmology, Shengjing Hospital, China Medical University, Shenyang 110003 Liaoning Province, China.
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)