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Abstract #24443 Published in IGR 11-3
Effect of serum containing qingguang'an grains on pressurized human trabecular cells activity
Peng Q-H; Zeng Z-C; Li B; Zhuo Y-HInternational Journal of Ophthalmology 2009; 9: 839-842
AIM: To observe the effects of serum containing qingguang'an grains on the activity of pressurized human trabecular cells cultured in vitro, and to investigate the mechanism of action in preventing and reducing the as census of postop intraocular pressure on primary open angle glaucoma. METHODS: The qingguang'an grains-contained serum was prepared, and the pressurized human trabecular cells were incubated with serum containing 2.5 times, 5 times, 10 times and 20 times qingguang'an grains and 5 times yimaikang tablets, respectively, for 12 hours, 24 hours, and 36 hours. The cell morphology was observed and cell activity (OD) was detected by MTT. RESULTS: OD value of pressurized human trabecular cells is concerned with concentration and action time of qingguang'an grains. On OD value, 5 times, 10 times and 20 times qingguang'an Grains groups were higher than blank group (P < 0.01), 10 times and 20 times qingguang'an grains groups were higher than yimaikang Tablets group (P < 0.01), but no conspicuous different between 5 times qingguang'an grains groups and 5 times yimaikang Tablets group; OD value of the cells cultured in qingguang'an grains-contained serum for 24 hours and 36 hours were higher than that cultured for 12 hours (P < 0.01), but no conspicuous different between 24 hours and 36 hours. CONCLUSION: Serum containing qingguang'an grains can raise pressurized human trabecular cells activity. LA: Chinese
Dr. Q.-H. Peng, Department of Ophthalmology, First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China. Pqhz_520@163.com
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
