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Abstract #24732 Published in IGR 11-4
Injury of gangliocyte in rat with chronic ocular hypertension
Sun J-F; Wang LInternational Journal of Ophthalmology 2009; 9: 1466-1468
AIM: To investigate the pressure-induced nerve damage in experimental chronic glaucoma model in rat. METHODS: Sixty-five adult male Wistar rats were rendered for elevation of intraocular pressure (IOP) by cauterizing 3 episcleral veins in the right eyes, and the left eyes were taken as the control group. 1 day; 1 week, 2, 3, 4, 6, 8, 10 weeks after cauterization of episcleral veins, 6 rats were sacrificed respectively. Retinal ganglion cells (RGC) retrograde labeling was completed with Fluorogold 24 hours before enucleation. The retina wholemount was performed, and the number of RGCs was counted. RESULTS: The mean IOP 1 day; 1 week, 2, 3, 4, 6, 8, 10 weeks after cauterization of episcleral veins was, 42.2 (plus or minus) 1.8mmHg, 37.9 (plus or minus) 2.3mmHg, 36.1 (plus or minus) 2.0mmHg, 33.4 (plus or minus) 2.1mmHg, 32.2 (plus or minus) 2.4mmHg, 30.1 (plus or minus) 2. OmmHg, 30.6 (plus or minus) 2.2 mmHg and 27.6 (plus or minus) 1.3mmHg, respectively. The success rate of high IOP model was 80.0%, 76.9%, 74.6%, 71.7%, 63.8%, 56.1%, 42.9% and 41.4% 1 day; 1, 2, 3, 4, 6, 8, 10 weeks after operation. The IOP and success rate decreased with time. There was no significant difference of RGC density between two groups at early stage (within 3 weeks after operation). At the fourth week, the RGC density in rats with high IOP was lower compared with those with normal IOP. The difference increased with time. CONCLUSION: Cauterization of episcleral veins induces durative, affirmative intraocular hypertension in rat. The pressure of model decreases with time. The loss of RGC increases with the duration of intraocular hypertension. LA: Chinese
L. Wang. Department of Ophthalmology, Ruijin Hospital, Medical College of Shanghai Jiao Tong University, Shanghai 200025, China. lwang@rjh.com.cn
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
