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Abstract #25571 Published in IGR 12-1
Expression of nitric oxide synthase in the lateral geniculate nucleus of rats with acute ocular hypertension
Hang H; Zhu C -H; Liu H; Ma Y -PInternational Journal of Ophthalmology 2009; 9: 2315-2317
AIM: To investigate the expression of nitric oxide synthase (NOS) in the retinas and lateral geniculate nucleus(LGN) of rats with acute ocular hypertension. METHODS: Four of 20 SD rats were randomly selected as blank control. The right eyes of the other 16 rats as control were perfused with sterile saline into the anterior chamber to elevate the intraocular pressure, and the maintaining time was 60 minutes. Rats were sacrificed at 0.5, 1,3 and 7 days after perfusion, and NOS expression in LGN were detected by NADPH-diaphorase histochemistry. RESULTS: The staining of NOS were localized in LGN of all the blank control and experimental group. In the LGN, NOS was mainly in the ventral part of LGN(vLGN). The count of NOS staining cells in the experimental group was significantly more than the blank control(P < 0.01). In the experimental group, NOS was significantly higher at 1 day after perfusion compared with other three experimental groups(P < 0.01). Numbers of NOS positive cells in other time points after perfusion had no statistical significance. CONCLUSION: NO not only plays an important role in normal function of eye, but also induces injury to the LGN of rats with acute ocular hypertension. LA: Chinese
H. Liu. Department of Ophthalmology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China. liuhu66@163.com
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.16 Chiasma and retrochiasmal central nervous system (Part of: 2 Anatomical structures in glaucoma)
3.6 Cellular biology (Part of: 3 Laboratory methods)