advertisement
Abstract #25800 Published in IGR 12-2
Measurement of retinal nerve fiber layer thickness with spectral domain optical coherence tomography
Wu H-J; Bao Y-Z; Ren Z-Q; Hou X-R; Liu G-DChinese Ophthalmic Research 2010; 28: 445-449
Background: The evaluation of optic nerve head and retinal nerve fiber layer ( RNFL) thickness is essential in diagnosis and follow-up of glaucoma. Optical coherence tomography (OCT) is one of the imaging techniques for glaucoma, and the new developed technique of spectral domain OCT with faster scan speed is expected to reduce RNFL measurement variability. Objective: The present study tried to evaluate the reproducibility of measurement method for determining peri-papillary retinal nerve fiber layer (RNFL) thickness with Spectralis spectral domain optical coherence tomography (SD-OCT). Methods: SD-OCT was used in the lateral eyes of 30 non-glaucoma subjects and 30 glaucoma patients for the measurement of peri-papillary RNFL thickness. The measurement of peri-papillary RNFL thickness was repeated for three times during the same operation using the follow-up function of Spectralis OCT. The reproducibility was evaluated based on the within-subject standard deviation (Sw) , coefficient of variation (CV) and intraclass correlation coefficient (ICC). Spearman ' s rank correlation coefficient analysis was used to assess the correlation of the standard deviation of RNFL thickness values obtained from 3 times of measuring values in each participant. Results: The CVs ranged from 1.44% (360(degrees) retina) to 2.58% ( temporal quadrant) in non-glaucomatous eye and from 1.73% ( 360(degrees) retina) to 3.24% (temporal quadrant) in glaucomatous eyes. The range of ICCs was from 0.977 of temporal quadrant to 0.990 of inferior-nasal sector in non-glaucomatous eyes and from 0.981 of temporal quadrant to 0.997 of inferior quadrant in glaucomatous eyes. The Sw value showed a range from 1.33 (mu)m of 360(degrees) retina to 2.36 (mu)m of superior-temporal sector in non-glaucomatous eyes and from 1.13 (mu)m of 360(degrees) retina to 2.26 (mu)m of superior-nasal sector in glaucomatous eyes. No significant correlations were found between RNFL thickness values and measurement variability for each participant ( P > 0.05 ). Conclusion: The fast scan speed and the tracking system allow an excellent reproducibility of Spectralis OCT in measuring peri-papillary RNFL thickness in both non-glaucoma subjects and glaucoma patients, indicating that SD-OCT is a reliable imaging technique for the evaluation of glaucomatous damage in a long-term follow up duration. LA: Chinese
H.-J. Wu. Department of Ophthalmology, Peking University, People's Hospital, Beijing 100044, China.
Classification:
6.9.2.2 Posterior (Part of: 6 Clinical examination methods > 6.9 Computerized image analysis > 6.9.2 Optical coherence tomography)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
