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Abstract #25805 Published in IGR 12-2
Neuroprotective effect of pigment epithelium-derived factor on retinal ganglion cells of rats with chronic intraocular hypertension
Lu B-J; Gao X-W; Wang R-F; Yan H; Wang B-RChinese Ophthalmic Research 2010; 28: 427-431
Background: The chronic ocular hypertension will cause the damage of retinal ganglion cells (RGCs). Research showed that effect of pigment epithelium-derived factor ( PEDF ) has the protective effect on the nerve system. Objective: The present study was to investigate the effect of PEDF on the RGCs in chronic intraocular pressure (IOP) eyes. Methods: The chronic ocular hypertension models were created in 24 eyes of 24 female adult SPF Sprague Dawley rats by cauterizing the episcleral veins to elevate the IOP to 31 mmHg. The another 12 matched rats only incised the bulber conjunctiva without cauterizing the episcleral veins as sham group. 2 (mu)L. (0.05 g/L) homologous recombination rat PEDF was intravitreously injected in 12 model rats and 12 sham rats after removal of 2 (mu)L vitreous body, and the equal amount of deionized water was intravitreously injected in another 12 model rats. The animals were sacrificed and the eye balls were cut with cryostat on and 14 days after injection, and the TUNEL and Fluoro-Jade (FJ) staining protocols were carried out to detect the apoptotic cells and degenerative neurons in the retina. The experiment followed the Regulations for the Administration of Affair Concerning Experimental Animals by State Science and Technology Commission. Results: The IOP was obviously increased in all of the model rats in comparison with the sham operation group (P < 0. 05). No significant difference was seen in IOP between 3 days and 14 days in each group ( P > 0. 05 ). The rate of TUNEL-positive cells was significantly increased in model + PEDF group and model + deionized water group in comparison with sham + PEDF group (both P <0. 05) ,and evidently decrease value was found in the rate of TUNEL-positive cells between model + PEDF group and model + deionized water group ( P < 0. 05 ) or between model + PEDF 3-day group and model + PEDF 14-day group (P < 0. 05). The rate of FJ-positive cells in retina followed the same pattern of TUNEL assay. Conclusion: Intravitreal administration of the PEDF has the protective effect on the ocular hypertension-damage of RGCs by reducing the apoptosis and degeneration of neuron. LA: Chinese
B.-J. Lu. Department of Ophthalmology, No. 474 Hospital of PLA, Urumqi 830013, China. lbingjian@126.com
Classification:
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
11.8 Neuroprotection (Part of: 11 Medical treatment)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
