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Abstract #25956 Published in IGR 12-2
Lactate dehydrogenase and oxidative stress activity in primary open-angle glaucoma aqueous humour
Jovanovic P; Zoric L; Stefanovic I; Dzunic B; Djordjevic-Jocic J; Radenkovic M; Jovanovic MBosnian journal of basic medical sciences 2010; 10: 83-88
Lactate dehydrogenase (LDH) and lactate are some of the hypoxy biochemical parameters. Extracellular activity of this enzyme increases under the condition of oxidative stress, since the cell integrity can be disrupted during the lipid peroxidation process. Subsequently that leads to the increase level of the lactic acid and lactic acid salts. The objective of this investigation is establishing the level of LDH, LDH1 (HBDH) and the lactate concentration in aqueous humour in patients with primary open-angle glaucoma. Biochemical analysis have been made by enzymatic-colometric method (lactate) and UV-kinetic method (LDH and HBDH) in aqueous humour of 30 patients (42 eyes) with primary open-angle glaucoma (POAG) and 30 patients (40 eyes) with cataract (the control group). The increased values of lactate and the activity of LDH and HBDH enzyme in aqueous humour of POAG patients in correlation with the control group are the results not only of oxidative stress but also of hypoxy and the mitochondry oxidative function (p P. Jovanovic. Ophthalmology Clinic, Clinical Center, Bulevar dr Zorana Djindjica 48, 18000 Nis, Serbia. 3.9 Pathophysiology (Part of: 3 Laboratory methods)Classification:
2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
3.7 Biochemistry (Part of: 3 Laboratory methods)
