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Abstract #26192 Published in IGR 12-2
Aqueous humor endothelin-1 (Et-1), vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) levels in Mexican glaucomatous patients
Iwabe S; Lamas M; Vásquez Pélaez CG; Carrasco FGCurrent Eye Research 2010; 35: 287-294
PURPOSE: To evaluate the concentration of endothelin-1, vascular endothelial growth factor, and cyclooxygenase-2 in aqueous humor from normal and glaucomatous human patients. MATERIAL AND METHODS: Concentrations of these proteins were measured using ELISA kits in 83 patients (30 presenting for cataract surgery and 53 with glaucoma). RESULTS: Endothelin-1, vascular endothelial growth factor, and cyclooxygenase-2 were detected in all samples. The ages of the patients with glaucoma (64.51 +/- 17.51 years) and cataracts subjects (59.30 +/- 19.15 years) were similar. The endothelin-1 concentration in cataracts patients (48.55 +/- 9.50 pg/ml) was statistically different when compared to endothelin-1 concentration in aqueous humor from primary open-angle glaucoma (107.94 +/- 12.20 pg/ml) and neovascular glaucoma (114.68 +/- 25.50 pg/ml) (p < 0.05). No statistical difference was observed in normal tension glaucoma group (69.14 +/- 52.80 pg/ml) (p > 0.05). Vascular endothelial growth factor concentration was higher in neovascular glaucoma (81.84 +/- 6.40 pg/ml), being the difference statistically significant when compared with the other groups (p < 0.0001). Cyclooxygenase-2 levels showed no statistical difference between cataract groups and any other glaucoma group (p > 0.05). No effect of age, gender, or previously medication of aqueous humor concentration of these proteins could be detected (p > 0.05). CONCLUSION: In this study we observed increased levels of endothelin-1 in aqueous humor from patients with primary open-angle glaucoma and neovascular glaucoma, and increased levels of vascular endothelial growth factor only in neovascular glaucoma patients, raising a possible connection between endothelin-1 and vascular endothelial growth factor in the role of some types of glaucoma. Regarding cyclooxygenase-2 levels detected, perhaps indicate that low values of cyclooxygenase-2 are necessary for normal functions of the eye.
Pharmacobiology Department, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Mexico City, Mexico.
Classification:
3.7 Biochemistry (Part of: 3 Laboratory methods)
2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
