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Abstract #26879 Published in IGR 12-3
Focused Conference Group: P17 - Newapproaches and targets in psychiatry description of a new method of primary culture of bovine retinal ganglion cells
Palmero M; Company MA; Maneu V; Formigos JABasic and Clinical Pharmacology and Toxicology 2010; 107: 511
The retinal ganglion cells (RGC) are the primary cell type injured in a variety of diseases of the optic nerve, including glaucoma and optic neuritis. In order to study cellular and/or molecular pathways culminating in the death of RGCs, it is essential to establish the culture of RGC. Different methods have been described in rat retinal ganglion cells in order to increase survival time in culture, increase the number of RGC in a neural retina culture or extensive neurite outgrowth (Barres BA and Chun LLY. Neuroprotocols. 1993. Vol 2: 201-204.; Shoge K et al. Neuroscience Letters. 1999. Vol 259: 111-114). We have developed a new technique to culture retinal ganglion cells from bovine retinas. In contrast to other techniques, this is a direct and less aggressive method. We have obtained a stable culture that allows the survival of the cells in culture for 15 days and extensive neuritis outgrowth. This cell culture system may be used for future studies of survival or degeneration of bovine RGC and the effect of different drugs, under normal culture conditions and under adverse conditions, such as those that mimic glaucoma o neuronal degeneration.
M. Palmero. University of Alicante, Faculty of Science, Department of Optic, Pharmacology and Anatomy, San Vicente del Raspeig, AlicanteSpain.
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)