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Abstract #26903 Published in IGR 12-3
Matrix metalloproteinase 9 expression: new regulatory elements
Surgucheva I; Chidambaram K; Willoughby DA; Surguchov AJournal of Ocular Biology, Diseases, and Informatics 2010; 1-12
Retinal ganglion cells apoptosis is linked to matrix metalloproteinase 9 (MMP-9) controlled changes of extracellular matrix. Abnormal expression of MMP-9 is associated with glaucomatous alterations. Thus, the knowledge of MMP-9 regulation is important for the understanding the pathogenesis of glaucoma. Here, we investigated the role of 3'-untranslated regions (3'-UTR) and microRNAs in MMP-9 regulation. We used in vitro mutagenesis and Luc reporter system to identify regulatory elements in the 3'-UTR of MMP-9. microRNAs were analyzed by qRT-PCR, and their role was investigated with inhibitors and mimics. We identified targets for miRNAs in 3'-UTR of MMP-9 involved in the regulation of MMP-9 expression. We then isolated miRNAs from the optic nerve A7 astrocytes and 293 T cells and confirmed the role of mi340 in the regulation using specific inhibitors and mimics. The results obtained show a new miRNA-mediated mechanism of MMP-9 expression regulation.
A. Surguchov. Laboratory of Retinal Biology, VA Medical Center, 4801 E Linwood Blvd, Kansas City, 64128, MO, United States. asurguchov@kumc.edu
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
2.15 Optic nerve (Part of: 2 Anatomical structures in glaucoma)
