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Abstract #27281 Published in IGR 12-4
Expression of glucocorticoid receptors (alpha) and (beta) in mononuclear cells of peripheral blood in patients with glucocorticoid-induced ocular hypertension
Li G-G; Zhang H; Liu R; Xia Y; Bi J-JChinese Journal of Ophthalmology 2009; 45: 21-25
Objective: To study the expression of glucocorticoid receptors ((alpha) and (beta)) in mononuclear cells of peripheral blood (PBMCs) in patients with glucocorticoid-induced ocular hypertension (GIOH) and the relationship between of the expression of GR(alpha) and GR(beta) in PBMCs and its susceptibility to GIOH. Methods: Case control study. Thirty-two patients with anaphylactic conjunctivitis were enrolled in this study. All of whom were received topical application of 0.1% dexamethasone. The patients with the elevation of intraocular pressure (IOP) over 8 mm Hg (1 mm Hg = 0.133 kPa) or higher within two weeks were defined as GIOH and the others were considered as control group. There were no statistical difference in sex, average age, and baseline IOP between two groups. Each group consisted of sixteen patients. GR(alpha) and GR(beta) mRNA (relative value) in PBMCs were detected with semi-quantitative reverse transcriptional polymerase chain reaction (RT-PCR), GR(alpha) and GR(beta) protein (positive ratio) in PBMCs examined with fluorescent labelling flow cytometry. Results: The GR(alpha) mRNA/GAPDH ratio of GIOH group was significantly (t =3. 872,P <0. 05) higher than that of control group (1.152 (plus or minus) 0.057 vs 1.048 (plus or minus) 0.031). The GR(beta) mRNA/GAPDH of GIOH group was lower than that of control group's (1.055 (plus or minus) 0.034 vs 1. 063 (plus or minus) 0.035), but was not statistically different (t = 0.419, P > 0.05). GR(alpha) positive percentage was significantly (t = 8.513 , P < 0.01) higher in PBMCs of GIOH group than that of the control group (93. 10 (plus or minus) 7. 35) % vs (46. 00 (plus or minus) 13.11) %, whereas GR(beta) positive percentage was significantly (t = 4.842 P < 0.01) lower in PBMCs of GIOH group than that in control group (12.50 (plus or minus) 4.18) % vs (23.83 (plus or minus) 3.92) %. Conclusions: The higher expression of GR(alpha) in PBMCs in patients may have an implication of susceptibility to GIOH and warrant further investigation. LA: Chinese
H. Zhang. Department of Ophthalmology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China. dr_zhanghong@sina.com
Classification:
9.4.1 Steroid-induced glaucoma (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders)
