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Abstract #27282 Published in IGR 12-4
The influence of glaucoma filtration surgery on anterior chamber associated inmune deviation in mice
Xu J-J; Sun X-HChinese Journal of Ophthalmology 2009; 45: 32-37
Objective: To study glaucoma infiltration surgery induced the anterior chamber associated immune deviation (ACAID) by with or without anterior chamber (AC) implantation of foreign epithelium removed corneal graft in mice. Methods: In this study, fifty Wistar mice were randomly divided into five groups (ten in each group) and additional five SD and one wistar mouse were used as the providers of epithelium removed corneal grafts to induce ACAID as follows: Group A: corneal grafts from Wistar mouse were implanted into the AC 1 week after the infiltration surgery. Group B: the spleen cells were injected into the nape of neck 1 week after the glaucoma infiltration surgery. In group C, D, and E, the corneal grafts from SD mice were implanted into the AC at 1 week, 4 week, and 8 week after the glaucoma infiltration surgery, respectively. To induce the delayed type hypersensitivity(DTH), spleen cells were injected into the right ear pinna 1 week after the neck injection in group B, 2 weeks after the AC implantation of corneal grafts in other groups. At the same time points as the induction of DTH, heart blood was collected to detect the concentration of IL-4 and IL-10. The spleens were removed to evaluate the expression of the GATA-3 mRNA by RT-PCR. The eyeballs were enucleated and used to evaluate the histopathological changes. Results: (1) The DTH were found in group B, C, D, and E with corneal grafts of SD, but not in group A with that of Wistar. (2) The serum concentrations of IL-4 and IL-10 were statistically different in group C, D, and E (F = 49. 124,6. 336;P <0.01). The concentrations of IL-4 and IL-10 were significantly (P = 0. 002) lower in group D than those in group C and E (3. 759 (plus or minus) 0. 250 vs 5. 916 (plus or minus) 0. 500 or 4. 566 (plus or minus) 0. 518, and 17. 170 (plus or minus) 3. 943 vs 44. 447 (plus or minus) 17. 167 or 35. 643 (plus or minus) 21. 233 (mu)g/L, n = 10, respectively) , which showed a biphase response fluctuation of ILs. (3) The GATA-3 mRNA was obviously up-regulated in group D and E. (4) The exudation and a few inflammatory cells in the AC were observed in group C, but not in group D and E. Conclusions: The eye with the glaucoma infiltration surgery results in a temporary incapable of inducing ACAID, which is gradually recovered following the diminish of intraocular inflammation and the increase of levels of IL-4, IL-10 and GATA-3 mRNA. LA: Chinese
X.-H. Sun. Department of Ophthalmology, Eye and ENT Hospital, Fudan University, Shanghai 200031, China. xhsun@shmu.edu.cn
Classification:
12.8.1 Without tube implant (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
3.10 Immunobiology (Part of: 3 Laboratory methods)
