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Abstract #27363 Published in IGR 12-4
CO(2) laser-assisted filtering surgery in rabbits intraocular hypertension model
Yu P-P; Xu Y-S; Liu A-H; Zhao Y; Li Y-XChinese Ophthalmic Research 2010; 28: 628-631
Background: Although the conventional anti-glaucoma surgery can reduce intraocular pressure (IOP) to some extent, some postoperative complications such as hyphema, shallow anterior chamber and low IOP often occur at early stage, and fibrous scar formation on filtration tract also appeared at the late stage after operation. Objective: The aim of this study was to investigate the feasibility and validity of CO(2) laser-assisted filtering surgery to treat intraocular hypertension model. Methods: The ocular hypertension models were included by the posterior chamber injection of (alpha)-chymotrypsin in bilateral eyes of 20 adult Japanese white rabbits. The ultrapulse CO(2) laser-assisted filtering surgery was performed in the right eyes of rabbits and the conventional filtering surgery was taken in the left eyes. In laser group, CO(2) laser was emitted on the scleral flap beneath scleral bed to perform the trabeculectomy with the average power 1-2 W, pulse width 0.1 ms, frequency 100 Hz, and meanwhile iris radical excision was executed with the average power 4-5 W, pulse width 0.8 ms at the frequency of 100 Hz. The gradation of anterior chamber reaction and type of filtering bleb were assessed under the slim lamp, and intraocular pressure (IOP) was measured using Perkins applanation tonometer. The pathological examination of eyeballs was curried out in different time points after operation. Results: Anterior chamber reaction was more mild in laser group than conventional surgery group. A statistically significant difference was found in the numbers of eyes with anterior chamber response between two groups in 1, 3, 7 days after operation (P < 0.05). Thirty-nine eyes in both groups appeared the functional filtering bleb in 3 days after operation, and the proportion of functional filtering bleb was decreased significantly in conventional operative group in 30 days after operation, showing a significant difference between these two groups (P < 0.05). The IOP was gradually elevated in both groups with the prolong of postoperative time, especially in conventional operative group, presenting considerably differences between two groups from 14 days through 60 days after operation (P < 0.01). Histopathology examination showed that the proliferation of fibroblasts was slight in laser group compared with conventional operative group in the third day after operation, and the filtering trace was open till 60 day in laser group, but in conventional operative group, the filtering trace was close. The bleeding and inflammatory exudates were obvious in conventional operative group in comparison with laser group. Conclusion: Applying ultrapulse CO(2) laser to performe the trabeculectomy in rabbits with intraocular hypertension model is safe and feasible. LA: Chinese
P.-P. Yu. Tianjin Medical University Eye Center, Tianjin 300070, China. yupengpeng1983@163.com
Classification:
12.8.1 Without tube implant (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
12.4 Laser trabeculoplasty and other laser treatment of the angle (Part of: 12 Surgical treatment)
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
