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Abstract #27648 Published in IGR 12-4
The role of T-cell subsets and Natural Killer Lymphocytes in the Pathogenesis of Primary Open Angle Glaucoma
Fahmy IA; Amer AK; EL-Ghaffar NAMacedonian Journal of Medical Sciences 2010; 3: 307-313
Background. The aim of the work is to emphasize the role of lymphocyte subsets; T-helper (Th), Tcytotoxic/suppressor (Tc/s) and natural killer (NK) and their secreted cytokines found in the peripheral blood of patients suffering from glaucoma; primary open angle glaucoma (POAG) and normal tension glaucoma (NTG) in an attempt to find out the possible change met within such patients. Subjects and Methods. This study included 36 patients diagnosed as glaucoma of different etiologies including both intra ocular pressure (IOP) dependant glaucoma (19 patients) and IOP independent glaucoma (17 patients) and compared to 20 healthy subjects of matched age and sex as reference subjects. Lymphocyte subsets (Th, Tc/s & NK) were analyzed in the peripheral blood of POAG and NTG cases and reference subjects by flow cytometry and the results were expressed as percentage of lymphocytes that express (CD4+, CD8+ and CD16+56+). The cytokines; interleukin-2 (IL-2), interferongamma (IFN-gamma) and interleukin-10 (IL-10) were analyzed in the serum of POAG and NTG patients and reference subjects using ELISA technique. Results. The results of this study revealed a significant increase of the percentage of CD4+ and CD8+ cells, significant decrease in CD4+/CD8+ ratio in both POAG and NTG patients when compared to reference subjects. There was significant increase in the mean serum level of IL-10 in POAG and NTG patients when compared to reference subjects. Conclusion. This results support the hypothesis that the delicate intraocular balance of Th1/Th2 immune response, with the predominance of Th2, breaks down in glaucoma. (copyright) 2010 Fahmy IA.
N. A. EL-Ghaffar. National Research Center, Clinical and chemical pathology, Tahreer street, 126220 Dokky, Cairo, Egypt. abdelghaffarn@hotmail.com
Classification:
3.10 Immunobiology (Part of: 3 Laboratory methods)
