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Abstract #27649 Published in IGR 12-4
Rapid and noninvasive imaging of retinal ganglion cells in live mouse models of glaucoma
Tosi J; Wang NK; Zhao J; Chou CL; Kasanuki JM; Tsang SH; Nagasaki TMolecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging 2010; 12: 386-393
PURPOSE: We report a noninvasive method for the monitoring of retinal ganglion cell (RGC) survival in live mice utilizing standard fluorescence microscopy. PROCEDURES: Transgenic mice expressing cyan fluorescent protein (CFP) under the regulation of an RGC-specific promoter Thy1 were used in this study. RESULTS: We established that Thy1-CFP expression is a quantitative reflection of the number of surviving RGCs, the fluorescence emission is stable for at least a year and that the loss of fluorescence correlates directly to glaucomatous damage. In high pressure glaucoma model, the peripheral retina is preferentially affected. CONCLUSIONS: Our live-imaging technique allows for the longitudinal assessment of RGC survival from the same animal. Noninvasive monitoring of neuronal cell death and survival is a powerful technique that would allow investigators to validate new potential glaucoma therapy based on neuroprotection.
J. Tosi. Bernard and Shirlee Brown Glaucoma Laboratory, Departments of Ophthalmology, Pathology and Cell Biology, Columbia University, 630 West 168th Street, Box 18, New York, NY 10032, USA.
Classification:
3.13.3 RGC Imaging (Part of: 3 Laboratory methods > 3.13 In vivo imaging)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
