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Abstract #28118 Published in IGR 13-1
Oxidative DNA damage and total antioxidant status in glaucoma patients
Sorkhabi R; Ghorbanihaghjo A; Javadzadeh A; Rashtchizadeh N; Moharrery MMolecular Vision 2011; 17: 41-46
Purpose: To evaluate DNA damage markers and the antioxidant status of serum and aqueous humor in glaucoma patients. Methods: Aqueous humor and serum samples were obtained at the time of surgery from 28 patients with glaucoma and 27 patients with cataracts. Total antioxidant status (TAS) and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels of all samples were determined by spectrophotometric and enzyme-linked immunosorbent assay methods. Results: Aqueous levels of 8-OHdG were higher in glaucoma patients than in the cataract group (4.61(plus or minus)2.97 ng/ml versus 1.98(plus or minus)0.70 ng/ml, p=0.002). Serum levels of 8-OHdG were also higher in glaucoma patients than in the cataract group (17.80(plus or minus)8.06 ng/ml versus 13.63(plus or minus)3.54 ng/ml, p=0.046). The TAS levels of serum (0.55(plus or minus)0.13 mmol/lit versus 0.70(plus or minus)0.14, p=0.001), and aqueous humor (0.23(plus or minus)0.13 mmol/lit versus 0.34(plus or minus)0.15, p=0.001) in glaucoma patients were lower than in cataract patients. Conclusions: Our findings provide evidence that oxidative DNA damage increases and TAS decreases in the serum and aqueous humor of glaucoma patients. These findings support the hypothesis that the formation of reactive oxygen species and/or a decrease in TAS may have an important role in the pathogenesis of glaucoma. (copyright) 2011 Molecular Vision.
A. Ghorbanihaghjo. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz 51664, Iran. ghorbaniamir@hotmail.com
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
3.7 Biochemistry (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)