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Abstract #3400 Published in IGR 4-2
Expression of tissue inhibitors of metalloproteinase in monkey trabecular meshwork cells and ciliary muscle cells
Jin M; Wu JChinese Journal of Ophthalmology 2002; 38: 298-301
OBJECTIVE: To identify tissue inhibitors of metalloproteinase (TIMP) released by normal monkey trabecular meshwork cells and ciliary muscle cells in vitro, and to detect the roles of matrix metalloproteinase (MMP) and TIMP in trabecular outflow and uveoscleral outflow in normal eyes. METHODS: The expression of TIMP and MMP in monkey trabecular meshwork and ciliary muscle cultures was detected by reverse zymography technology. RESULTS: (1) TIMP-1, TIMP-2 and TIMP-3 were found in both trabecular meshwork and ciliary muscle cultured cells. (2) The ratio of MMP/TIMP in the trabecular meshwork cells was much higher than that in ciliary muscle cells. CONCLUSIONS: The different ratio of MMP/TIMP in these two types of cells suggests that the capability of degrading extracellular matrix in trabecular meshwork cells is much higher than that in ciliary muscle cells, which may be one of the reasons why conventional trabecular outflow is stronger than uveoscleral outflow. LA: Chinese
Dr. M. Jin, Department of Ophthalmology, The First Clinical College, China Medical University, Shenyang 110001, China. jin-m@21cn.com
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
2.9 Ciliary body (Part of: 2 Anatomical structures in glaucoma)
3.4 Molecular genetics (Part of: 3 Laboratory methods)