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Abstract #3615 Published in IGR 4-2
Determination of betaxolol in human aqueous humour by high-performance liquid chromatography with fluorescence detection
Dulger B; Basci NE; Sagdic Yalvac I; Temizer AJournal of Chromatography. B. Analyt Technol Biomed Life Sciences 2002; 772: 179-183
A reversed-phase high-performance liquid chromatographic method is described for the determination of betaxolol in human aqueous humor. Betaxolol and the internal standard metoprolol were extracted with cyclohexane and separated on a reversed-phase column (Luna C(18), 250 x 4.6 mm, 5 μm) with a mobile phase containing acetonitrile-phosphate buffer (40:60, v/v) at a flow-rate of 0.8 ml/min. The column effluent was monitored with a fluorescence detector at 227 nm (excitation) and 301 nm (emission). The retention times for metoprolol and betaxolol were 3.55 and 5.63 minutes, respectively. The recovery from aqueous humor was found to be 71.6% for betaxolol at 1.25 μg/ml. The within-day and day-to-day accuracy values were in the range of 96.17-105.2% for betaxolol at 0.1, 4 and 12 μg/ml (n = 6), within-day and day-to-day precision values were less than 10% for betaxolol at the concentrations given above. The detection limit corresponding to the signal-to-noise ratio of 3:1 was 15 ng/ml. The presented method was suitable for measuring betaxolol levels in human aqueous humor samples obtained from patients after topical administration.
Dr. B. Dulger, Hacettepe University, Faculty of Pharmacy, Department of Analytical Chemistry, 06100 Ankara, Turkey. berrakdulger@hotmail.com
Classification:
11.3.4 Betablocker (Part of: 11 Medical treatment > 11.3 Adrenergic drugs)
