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Abstract #3674 Published in IGR 4-2
Anti-allergic drugs inhibit the proliferation of human Tenon's capsule fibroblast and maintain the experimental filtering blebs on rabbit eyes
Kondo S; Yamamoto M; Takeuchi A; Suzuki H; Iwaki MNippon Ganka Gakkai Zasshi 2002; 106: 325-331
PURPOSE: To examine whether tranilast and ketotifen fumarate inhibit the growth of human tenon's capsule fibroblasts and maintain experimental filtering blebs on rabbit eyes. METHODS: Human tenon's capsule fibroblasts were grown in Dulbecco's modified eagle's medium with 10% fetal calf serum and growth was measured with 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and direct counting of cell number. Production of collagen and transforming growth factor-β1 (TGF-β1) was measured with corresponding enzyme-linked immunosorbent assay (ELISA). In experimental filtering surgery performed on rabbit eyes, the effects of the topical drugs on intraocular pressure and on maintenance of the filtering blebs were observed. RESULTS: Both tranilast and ketotifen inhibited the growth of the fibroblasts and half-inhibitory concentrations were 200 and 70 μm, respectively. Low concentration (10 μm) of tranilast and ketotifen decreased the synthesis of collagen but neither drug had any obvious effect on TGF-β1 production. Both drugs prolonged the life of the experimental filtering bleb significantly (12.5 ± 3.2 (mean ± standard deviation) days, 13.9 ± 3.4 days, respectively; control, 10.5 ± 2.4 days). CONCLUSIONS: Tranilast and ketotifen inhibited the growth of human tenon's capsule proliferation and are capable of maintaining experimental filtering blebs in rabbit eyes. LA: Japanese
Dr. S.Kondo, Department of Ophthalmology, Aichi Medical University, 21 Iwasaku, Karimata, Nagakute-cho, Aichi-gun, Aichi 480-1195, Japan
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
