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Abstract #45582 Published in IGR 13-2
Fluorophotometric and tonometric evaluation of ocular effects following aqueocentesis performed with needles of various sizes in dogs
Allbaugh RA; Roush JK; Rankin AJ; Davidson HJAmerican Journal of Veterinary Research 2011; 72: 556-561
Objective-To measure ocular effects (blood-aqueous barrier breakdown and intraocular pressure [IOP]) following aqueocentesis performed with needles of various sizes in dogs. Animals-28 healthy adult dogs. Procedures-24 dogs underwent unilateral aqueocentesis (24 treated eyes and 24 contra-lateral untreated eyes); 25-, 27-, or 30-gauge needles were used in 3 treatment groups (n = 8/group). Four dogs were untreated controls. Aqueocentesis was performed during sedation and topical anesthesia. Anterior chamber fuorophotometry was performed before and after aqueocentesis on day 1. On days 2 through 5, sedation and fuorophotometry were repeated. Intraocular pressure was measured with a rebound tonometer at multiple time points. Results-Aqueocentesis resulted in blood-aqueous barrier breakdown detected via fuorophotometry in all treated eyes, with barrier reestablishment by day 5. On day 2, the contralateral untreated eyes of all 3 groups also had signifcantly increased fuorescence. Use of a 25-gauge needle resulted in a signifcant increase in treated eyes' anterior chamber fuorescence on days 3 and 5 as well as a signifcant increase in IOP 20 minutes following aqueocentesis, compared with the other treatment groups. Conclusions and Clinical Relevance-Aqueocentesis performed with a 25-gauge needle resulted in the greatest degree of blood-aqueous barrier breakdown and a brief state of intraocular hypertension. Use of a 27- or 30-gauge needle is recommended for aqueous paracentesis. A consensual ocular reaction appeared to occur in dogs following unilateral traumatic blood-aqueous barrier breakdown and may be of clinical importance.
R. A. Allbaugh. Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, United States. Email: rachelallbaugh@yahoo.com
Classification:
15 Miscellaneous
5.3 Other (Part of: 5 Experimental glaucoma; animal models)
