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Abstract #45610 Published in IGR 13-2
Effects of glaucoma medications and preservatives on cultured human trabecular meshwork and non-pigmented ciliary epithelial cell lines
Ammar DA; Kahook MKBritish Journal of Ophthalmology 2011;
Aims: We investigated the potential cytotoxicity of various topical ophthalmic glaucoma formulations containing different preservatives in cultured human trabecular meshwork (TM) and non-pigmented ciliary epithelial (NPCE) cell lines. Methods: We tested 0.004% travoprost preserved with either 0.015% benzalkonium chloride (BAK), sofZia or 0.001% Polyquad (PQ); and 0.005% latanoprost preserved with 0.020% BAK. We also tested a range of BAK concentrations in balanced salt solution (BSS). TM cells were treated for 10 min at 37(degrees)C with solutions diluted 1:10 to mimic the reduced penetration of topical preparations to the anterior chamber. Viability was determined by the uptake of the fluorescent vital dye calcein-AM (n=6). Results: BAK solutions (diluted 1:10) demonstrated a dose-dependent reduction in cell viability in both cell types (TM and NPCE). With a 1:10 dilution of 0.020% BAK, there were significantly more living NPCE cells (89(plus or minus)6%) than TM cells (57(plus or minus)6%; p<0.001). In TM cells, travoprost + BAK had statistically fewer live cells (83(plus or minus)5%) than both travoprost + sofZia (97(plus or minus)5%) and travoprost + PQ (97(plus or minus)6%; p<0.05). Compared with BSS-treated NPCE cells, travoprost had statistically fewer live cells (p<0.05) when preserved with BAK (856(plus or minus)16%), sofZia (91(plus or minus)6%) or PQ (94(plus or minus)62%). Conclusions: These results demonstrate that substitution of BAK from topical ophthalmic drugs results in greater viability of cultured TM cells, the cells involved in the conventional outflow pathway. Cultured NPCE, responsible for aqueous inflow, appear more resilient to BAK.
M.K. Kahook. Rocky Mountain Lions Eye Institute, Department of Ophthalmology, University of Colorado Denver, 1675, . Email: malik.kahook@gmail.com
Classification:
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
2.9 Ciliary body (Part of: 2 Anatomical structures in glaucoma)
11.16 Vehicles, delivery systems, pharmacokinetics, formulation (Part of: 11 Medical treatment)
