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Abstract #45633 Published in IGR 13-2
Evaluation of rebound tonometry in non-human primates
Elsmo EJ; Kiland JA; Kaufman PL; McLellan GJExperimental Eye Research 2011; 92: 268-273
To determine the accuracy and reproducibility of intraocular pressure (IOP) measurements obtained with the TonoVet((registered trademark)) rebound tonometer in cynomolgus macaques and to determine the effects of corneal thickness on measurements obtained by the TonoVet((registered trademark)). The anterior chambers of both eyes of anesthetized monkeys were cannulated with branched 23-G needles; one branch was connected to a vertically adjustable reservoir and the other to a pressure transducer. IOP was increased by 5 mmHg increments and then decreased by 10 mmHg decrements. IOP was measured using the TonoVet((registered trademark)) at each increment and decrement by 2 independent observers and at every other increment and every decrement by a single observer using 'minified' Goldmann applanation tonometry. Central corneal thickness was measured with a PachPen(TM) ultrasonic pachymeter. TonoVet((registered trademark)) readings correlated well with manometric IOP (slope = 0.972, r(2) coefficient = 0.955). No significant differences were observed when comparing eyes or operators; however there was a non-significant trend for TonoVet((registered trademark)) readings taken in right eyes to be closer to manometric IOP than those taken in left eyes. The TonoVet((registered trademark)) had a non-significant tendency to underestimate manometric IOP. TonoVet((registered trademark)) readings obtained during the decremental phase of the experiment were significantly closer (p < 0.004) to manometric IOP than those obtained during the incremental phase. Central corneal thickness significantly increased (p < 0.0001) over the course of the experiment. The TonoVet((registered trademark)) rebound tonometer is a reliable and accurate tool for the measurement of IOP in cynomolgus macaques. This tonometer has several advantages, including portability, ease of use, and brief contact with the corneal surface making topical anesthetics unnecessary.
G.J. McLellan. F4/341 Clinical Sciences Center, 600 Highland Avenue, Madison, WI 53704, United States. Email: mclellan@svm.vetmed.wisc.edu
Classification:
6.1.1 Devices, techniques (Part of: 6 Clinical examination methods > 6.1 Intraocular pressure measurement; factors affecting IOP)
5.2 Primates (Part of: 5 Experimental glaucoma; animal models)
