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Abstract #45711 Published in IGR 13-2
A novel method for the induction of experimental glaucoma using magnetic microspheres
Samsel PA; Kisiswa L; Erichsen JT; Cross SD; Morgan JEInvestigative Ophthalmology and Visual Science 2011; 52: 1671-1675
See also comment(s) by John Morrison •
PURPOSE. The development of a method for the sustained elevation of intraocular pressure in experimental glaucoma based on the anterior chamber injection of paramagnetic microbeads. METHODS. Unilateral glaucoma was induced in adult male Norwegian Brown rats by the injection of paramagnetic polystyrene microspheres. A handheld 0.45 Tesla magnet was used to draw the beads into the iridocorneal angle to impede aqueous drainage via the trabecular meshwork. RESULTS. Elevated intraocular pressures (IOPs) were induced in 61 rats, resulting in a mean elevation of 5.8 mm Hg (plus or minus) 1.0 (SEM) relative to the contralateral control eye. The mean duration of sustained IOP elevation (defined as >5 mm Hg relative to the control eye for at least 7 consecutive days) after a single injection was 12.8 days (plus or minus) 0.9 (SEM, maximum duration 27 days). In all eyes, the visual axis remained clear from the time of injection, with minimal inflammation after injection. Retinal ganglion cell loss was determined in 21 animals (mean integral IOP, 194.5 mm Hg days (plus or minus) 87.5 [SEM]) as 36.4% (plus or minus) 2.4 (SEM) compared with the contralateral, untreated eye. CONCLUSIONS. The use of paramagnetic microbeads for the occlusion of the iridocorneal angle produces a sustained elevation of IOP with fewer injections and avoids the risk of visual axis occlusion. It represents a simple and effective method for the induction of experimental glaucoma.
J. E. Morgan. School of Optometry and Vision Sciences, Cardiff University, Maindy Road, Cardiff CF24 4LU, United Kingdom. Email: morganje3@cardiff.ac.uk
Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
