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Abstract #45803 Published in IGR 13-2
Long-term topical antiglaucoma medications cause enhanced Tenon's capsule fibroblast proliferation and abnormal TGF-(beta) and MMP expressions: Potential effects on glaucoma filtering surgery
Leng F; Liu P; Li H; Zhang JCurrent Eye Research 2011; 36: 301-309
Purpose: To investigate the changes in Tenon's capsule fibroblast (HTF) proliferation and their expressions of transforming growth factor-(beta) (TGF-(beta)) and matrix metalloproteinases (MMPs) in patients with long-term use of topical antiglaucoma medications, and to evaluate the potential effect of these medications on the outcome of glaucoma filtering surgery for primary open-angle glaucoma (POAG). Methods: HTFs isolated from the conjunctiva of ten POAG patients and ten control patients with cataract or retinal detachment were tested for their in vitro proliferation capacity. RT-PCR (reverse-transcription polymerase chain reaction) was performed to detect the expressions of TGF-(beta) and MMP mRNAs in the HTFs, and the expression of TGF-(beta)2 protein was detected immunohistochemically. Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of TGF-(beta)1 in the supernatant of the HTFs treated with the topical antiglaucoma agents. Results: The HTFs from the POAG patients showed significantly higher proliferative activity than those from the control patients (P=0.0013). The HTFs in the POAG group showed significantly higher expressions of TGF-(beta)2 and TGF-(beta)3 mRNA and lower MMP-9 mRNA than the control HTFs. MMP-3 mRNA was not detected in the HTFs in POAG groups, but lowly expressed in the control HTFs. The HTFs in the POAG group had a significantly higher expression of TGF-(beta)2 protein; TGF-(beta)1 protein was not detected in the control HTFs, but was highly expressed in the POAG group. Treatment of the HTFs with latanoprost resulted in a 4-fold increase in TGF-(beta)1 expression in both groups, which was comparable for the expression after the exposure. Conclusions: Long-term use of some topical antiglaucoma medications might be associated with enhanced HTF proliferation and abnormal expressions of TGF-(beta) and MMPs, which might have promoted scarring of the filtering blebs following glaucoma filtering surgery.
J. Zhang. Department of Ophthalmology, First Affiliated Hospital, Harbin Medical University, Harbin 150001, China. Email: zjyzn@hotmail.com
Classification:
2.1 Conjunctiva (Part of: 2 Anatomical structures in glaucoma)
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
11.16 Vehicles, delivery systems, pharmacokinetics, formulation (Part of: 11 Medical treatment)
