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Abstract #46491 Published in IGR 13-3
Hypoxia induces beta-amyloid in association with death of RGC-5 cells in culture
Li J; Dong Z; Liu B; Zhuo Y; Sun X; Yang Z; Ge J; Tan ZBiochemical and Biophysical Research Communications 2011; 410: 40-44
Beta-amyloid (A(beta)) derived from amyloid precursor protein (APP) has been associated with retinal degeneration in Alzheimer's disease (AD) and glaucoma. This study examined whether hypoxia exposure induces A(beta) accumulation in RGC-5 cells. While levels of APP mRNA and protein significantly increased in the cells, elevated abundance of A(beta) was also observed in cells and culture medium between 12 or 24 and 48h after 5% O(2) hypoxia treatment. Additionally, there is a close relationship between induction of APP and A(beta) and intracellular accumulation of ROS along with loss of mitochondrial membrane potential followed by the death of RGC-5 cells in culture under hypoxia. These results suggest a possible involvement of APP and A(beta) in the death of RGCs challenged by hypoxia.
J. Ge. UCI School of Medicine/Neurology, 100 Irvine Hall, Zot 4275, Irvine, CA 92697-4275, United States.
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
3.9 Pathophysiology (Part of: 3 Laboratory methods)
2.13 Retina and retinal nerve fibre layer (Part of: 2 Anatomical structures in glaucoma)
