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Abstract #46980 Published in IGR 13-3
Association of tumour necrosis factor-alpha -308 G/A polymorphism with primary open-angle glaucoma
Bozkurt B; Mesci L; Irkec M; Ozdag BB; Sanal O; Arslan U; Ersoy F; Tezcan IClinical and Experimental Ophthalmology 2011;
Background: Tumour necrosis factor-alpha (TNF-(alpha)) is an important proinflammatory cytokine driving axonal degeneration and retinal ganglion cell apoptosis in glaucoma. The aim of the study was to evaluate the association of TNF-(alpha) -308 G/A and -238 G/A polymorphisms with primary open-angle glaucoma (POAG). Design: A prospective, case-control study, university hospital setting. Participants: Eighty-six POAG patients and 193 healthy unrelated controls. Methods: TNF-(alpha) polymorphisms were screened by using direct gene sequencing. Main Outcome Measures: Frequency of TNF-(alpha) -308 G/A and TNF-(alpha) -238 G/A promoter polymorphisms in glaucoma and healthy subjects. Results: The frequencies of TNF-(alpha) -308 GA genotype and 'A' allele were higher in patients with POAG (22.1% and 12.2%, respectively) in comparison with the control group (10.9% and 6%, respectively) (P=0.046 and 0.02, respectively), with odds ratios of 2.45 (P=0.01, 95% CI=1.23-4.87) and 2.19 (P=0.013, 95% CI=1.18-4.08), respectively. Genotype distribution of the TNF-(alpha) -238 variants did not yield a statistically significant difference between the two groups (P=0.87). Conclusion: TNF-(alpha) -308 G/A polymorphism seems to be associated with POAG in Turkish population. However, population-based studies with large number of subjects and long-term follow-up are needed to verify the association of TNF-(alpha) -308 G/A polymorphism with glaucoma susceptibility.
B. Bozkurt. Selcuk University, Meram Medical Faculty, Department of Ophthalmology, Konya 42080, Turkey, .
Classification:
3.6 Cellular biology (Part of: 3 Laboratory methods)
3.4.2 Gene studies (Part of: 3 Laboratory methods > 3.4 Molecular genetics)
