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Abstract #47067 Published in IGR 13-3

Regulation of ocular adrenoceptor genes expression by 5-MCA-NAT: Implications for glaucoma treatment

Crooke A; Huete-Toral F; Martinez-Aguila A; Alarma-Estrany P; Pintor J
Pharmacogenet Genomics 2011; 21: 587-589

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We have demonstrated that 5-methoxycarbonylamino-N-acetyltryptamine (5-MCA-NAT), reduces intraocular pressure (IOP) in rabbits. In addition, we have reported a link between hypotensive effect of 5-MCA-NAT and sympathetic nervous system. Moreover, it is known that aqueous humour production is controlled by the activation of adrenoceptors (ADRs) present in the ocular ciliary epithelium. Thus, the aim of this study is to investigate if the hypotensive effect of 5-MCA-NAT is due to a regulation of ciliary ADR genes expression. To confirm this we followed the effect of 5-MCA-NAT on rabbit IOP for 144 consecutive hours. A sustained IOP reduction for up to 72 h (P<0.01) was seen. In addition, changes in ADRB2 and ADRA2A mRNA were measured in cultured rabbit nonpigmented ciliary epithelial cells. After 5-MCA-NAT treatment, a significant downregulation of ADRB2 and upregulation of ADRA2A was observed. These results provide the regulation of ADRs mRNA by 5-MCA-NAT.

J. Pintor. Departamento de Bioquimica y Biologia Molecular IV, E.U. Optica, Universidad Complutense de Madrid, C/Arcos de Jalon 118, Madrid 28037, Spain.

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Classification:

3.4.2 Gene studies (Part of: 3 Laboratory methods > 3.4 Molecular genetics)
3.8 Pharmacology (Part of: 3 Laboratory methods)

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