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Abstract #53934 Published in IGR 15-2
Intraocular pressure measurement in acepromazine-sedated mice
Chu ER; Kim EK; Gonzalez JM; Ko MH; Liew EC; Tan JCClinical and Experimental Ophthalmology 2013; 0:
Anaesthesia may affect steady state intraocular pressure (IOP) and its interpretation in glaucoma studies involving mice. Ketamine is a commonly used anaesthetic but it lowers mouse IOP.(1-5) An alternative such as gentle restraint (e.g. cone restraint) without anaesthesia has been used to avoid this undesirable effect,(1) but restraint itself can cause artifactual readings such as by artificially elevating episcleral venous pressure. To avoid this issue, non-anaesthetic sedation using acepromazine may be an alternative. Although acepromazine has been used to supplement rat behaviorial training for conscious IOP measurement,(3) to the best of our knowledge, IOP measurement under acepromazine sedation has not been reported in mice.
Doheny Eye Institute, 1450 San Pablo Street, Los Angeles, CA, 90033, USA; Department of Ophthalmology, Keck School of Medicine, University of Southern California, 1450 San Pablo Street, Los Angeles, CA, 90033, USA.
Full articleClassification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
6.1.1 Devices, techniques (Part of: 6 Clinical examination methods > 6.1 Intraocular pressure measurement; factors affecting IOP)