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Abstract #5585 Published in IGR 2-1
The similarity of protein expression in trabecular meshwork and lamina cribrosa: implications for glaucoma
Steeley HT Jr; English-Wright SL; Clark AFExperimental Eye Research 2000; 70:17-30
The purpose of the present investigation was to compare protein expression in various ocular cells and tissues including the human trabecular meshwork (TM) and the lamina cribrosa (LC). To conduct the comparisons, the authors primarily utilized autofluorography of one-dimensional (1D) and high resolution, two-dimensional (2D) polyacrylamide gels of proteins from radiolabelled tissues and cultured cells. Results from the investigations indicated that patterns of protein expression from TM and LC were the most similar among the ocular cells and tissues compared. Specifically, these autofluorographic 'fingerprints' indicated that proteins in TM and LC cultured cells and tissue were exceptionally similar (a) in band position and intensity (1D gels) and (b) in spot congruence (2D gels) as compared to other ocular cells and tissues. The authors conclude that the TM and the LC, two ocular tissues intimately linked to the pathogenesis of primary open-angle glaucoma, display remarkable similarity in protein expression. This finding may have implications for the molecular etiology of glaucoma.
Dr. H.T. Steely Jr, Glaucoma Research, R2-41 Alcon Laboratories, Inc., 6201 South Freeway, Fort Worth, TX 76134-2099, USA
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
2.14 Optic disc (Part of: 2 Anatomical structures in glaucoma)
