advertisement
Abstract #5630 Published in IGR 2-1
Effect of latrunculin-B on outflow facility in monkeys
Peterson JA; Tian B; Geiger B; Kaufman PLExperimental Eye Research 2000; 70: 307-313
Latrunculin-B (LAT-B), a macrolide derived from the marine sponge, Latrunculia magnifica, sequesters monomeric G-actin, leading to the disassembly of actin filaments in cultured cells. In this study, the authors determined the effect of LAT-B on outflow facility in living monkeys. Total outflow facility was measured by two-level constant pressure perfusion of the anterior chamber (AC) before and immediately after AC exchange infusion or two hours after topical application of LAT-B or vehicle. Both AC exchange infusion and topical application of LAT-B dose- and time-dependently increased outflow facility by two- to four-fold. Those findings suggest that pharmacological disorganization of the actin cytoskeleton in the trabecular meshwork by specific actin inhibitors such as LAT-B may be a useful anti-glaucoma strategy.
Dr. J.A. Peterson, Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison, WI, 53792-3220, USA
Classification:
5 Experimental glaucoma; animal models
11.14 Investigational drugs; pharmacological experiments (Part of: 11 Medical treatment)
