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Abstract #6256 Published in IGR 2-2
Experimental study of cytokines complex inhibitory effect on wound healing after glaucoma filtering operations: histopathological and immunochemical findings
Khoroshilova-Maslova IP; Gankovskaia LV; Andreeva LD; Erichev VP; Vasilenkova LV; Ilatovskaia LVVestnik Oftalmologii 2000; 116: 5-8
Experiments on a model of glaucoma filtering operation in 25 rabbits showed that the natural cytokine complex inhibited the operation wound healing in comparison with the control. The complex was prepared by an original method (culturing peripheral blood leukocytes) at the Immunology Department of the Russian State Medical University. Histopathological study revealed delayed formation of cicatricial tissue under the effect of cytokines. Decreased proliferation of fibroblasts and their decreased functional activity (decreased synthetic activity of glycosaminoglycanes and collagen) was detected in the cicatricial tissue. Immunohistochemical studies showed that in long-term periods (after three to eight months), type IV membrane collagen formed in new cicatricial tissue, while in the control, types III and I collagens predominated in the cicatricial tissue. The immunohistochemical structure of the cicatrix in the experiment correlated with the fine fibrous structure of cicatricial tissue, characterized by high permeability. Possible mechanisms of the inhibitory effect of the natural cytokine complex on the course of operation wound healing after glaucoma-filtering surgery are discussed. LA: Russian
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)