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Abstract #6616 Published in IGR 4-1
Effect of RU38486 on fibronectin expression in cultured human trabecular cells
Li F; Ye T; Wang N; Zheng HChinese Journal of Ophthalmology 2002; 38: 42
OBJECTIVE: To study the effect of RU38486 on fibronectin (FN) expression in human trabecular cells (HTCs) in vitro. METHODS: The trabecular specimens from human donors were firstly cultured and subcultured. Cultured cells were observed by light and electron microscopes. FN, laminin (LN) and neuron-specific enolase (NSE) in the extracellular matrix (ECM) of the cells were immunohistochemically stained with the LSAB method. The quantities of FN (optical density (A) means) affected by different concentrations of Dexamethasone (Dex) and RU38486 were measured by the indirect immunochemical methods associated with computer image analysis. RESULTS: According to the growing characteristics and morphological features, the cultured cells were identified as HTCs. Comparison of A means of FN induced by Dex and RU38486 was 10-6 mol/L Dex (12 days) > 10-7 mol/L Dex (12 d) > 10-7 mol/L Dex (5 d) > control group = 10-7 mol/L Dex (5 d) + 10-8 mol/L RU38486 (7 d). CONCLUSIONS: Dex can stimulate HTCs in vitro in order to secrete more FN. Bit RU38486 can reverse this high level of FN expression induced by Dex on a receptor level. The efficacy of RU38486 (glucocorticoid receptor antagonist) in reducing intraocular pressure in vivo should be confirmed by further studies.LA: Chinese
Dr. F. Li, Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China. champkee@163.net
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)
