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Abstract #6711 Published in IGR 4-1
Effect of palmitoleic acid on bradykinin-induced endothelium-dependent relaxation in isolated pig ciliary artery
Morf T; Beny JL; Flammer J; Haefliger IOKlinische Monatsblätter für Augenheilkunde 2002; 219:284-288
BACKGROUND: Endothelial-dependent relaxation has been reported to be impaired in some normal tension glaucoma patients. The present study investigates whether the gap junction uncoupling agent palmitoleic acid (PA) affects bradykinin-induced endothelium-dependent relaxation in isolated pig ciliary artery. MATERIAL AND METHODS: In a myograph system (isometric force measurement), vessels precontracted with the thromboxane A2 agonist U 46619 (approximately 0.1 μm) were relaxed by increasing concentrations (cumulative) of bradykinin (0.003-3 μm). Experiments were repeated in the presence of 100 μm L-NAME (inhibitor of nitric oxide formation) and/or 100 μm PA. Some experiments were conducted in vessels with a non-functional endothelium (intentionally and mechanically damaged). All experiments were conducted in the presence of 10 μM indomethacin (cyclooxygenase inhibitor). RESULTS: In a concentration-dependent manner, bradykinin evoked a relaxation (101 ± 2%) that was abolished in vessels with a non-functional endothelium (maximal relaxation: 7 ± 1 %, p < 0.001). In the presence of L-NAME, relaxations induced by bradykinin were almost completely inhibited (maximal relaxation: 25 ± 5 %, p < 0.001). Relaxations evoked by bradykinin were not significantly affected by PA (either in the presence or in the absence of L-NAME). CONCLUSIONS: The bradykinin-induced relaxation, known to be associated in porcine ciliary arteries with an electrical coupling between endothelial and smooth muscle cells, appears to be unaffected by the gap junction uncoupling agent palmitoleic acid. Further investigations are needed to understand the physiology of the endothelium-dependent ocular blood flow modulation that is considered to be dysregulated in some glaucoma patients.
Dr. T. Morf, Laboratory of Ocular Pharmacology and Physiology, University Eye Clinic Basel, Basel, Switzerland
Classification:
6.11 Bloodflow measurements (Part of: 6 Clinical examination methods)
11.7 Treatment of bloodflow (Part of: 11 Medical treatment)
