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Abstract #67581 Published in IGR 17-4
Preliminary investigation of multispectral retinal tissue oximetry mapping using a hyperspectral retinal camera
Desjardins M; Sylvestre JP; Jafari R; Kulasekara S; Rose K; Trussart R; Arbour JD; Hudson C; Lesage FExperimental Eye Research 2016; 146: 330-340
Oximetry measurement of principal retinal vessels represents a first step towards understanding retinal metabolism, but the technique could be significantly enhanced by spectral imaging of the fundus outside of main vessels. In this study, a recently developed Hyperspectral Retinal Camera was used to measure relative oximetric (SatO2) and total hemoglobin (HbT) maps of the retina, outside of large vessels, in healthy volunteers at baseline (N = 7) and during systemic hypoxia (N = 11), as well as in patients with glaucoma (N = 2). Images of the retina, on a field of view of ∼30°, were acquired between 500 and 600 nm with 2 and 5 nm steps, in under 3 s. The reflectance spectrum from each pixel was fitted to a model having oxy- and deoxyhemoglobin as the main absorbers and scattering modeled by a power law, yielding estimates of relative SatO2 and HbT over the fundus. Average optic nerve head (ONH) saturation over 8 eyes was 68 ± 5%. During systemic hypoxia, mean ONH saturation decreased by 12.5% on average. Upon further development and validation, the relative SatO2 and HbT maps of microvasculature obtained with this imaging system could ultimately contribute to the diagnostic and management of diseases affecting the ONH and retina.
École Polytechnique de Montréal, Institut de génie biomédical, Département de Génie électrique, 2900, boul. Édouard-Montpetit, Montréal, Qc, H3T 1J4, Canada. Electronic address: midesjardins@ucsd.edu.
Full articleClassification:
6.11 Bloodflow measurements (Part of: 6 Clinical examination methods)
6.8.2 Posterior segment (Part of: 6 Clinical examination methods > 6.8 Photography)
