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Abstract #68982 Published in IGR 18-1
Human aqueous humor proteome in cataract, glaucoma, and pseudoexfoliation syndrome
Kliuchnikova AA; Samokhina NI; Ilina IY; Karpov DS; Pyatnitskiy MA; Kuznetsova KG; Toropygin IY; Kochergin SA; Alekseev IB; Zgoda VG; Archakov AI; Moshkovskii SAProteomics 2016; 16: 1938-1946
Twenty-nine human aqueous humor samples from patients with eye diseases such as cataract and glaucoma with and without pseudoexfoliation syndrome were characterized by LC-high resolution MS analysis. In total, 269 protein groups were identified with 1% false discovery rate including 32 groups that were not reported previously for this biological fluid. Since the samples were analyzed individually, but not pooled, 36 proteins were identified in all samples, comprising the constitutive proteome of the fluid. The most dominant molecular function of aqueous humor proteins as determined by GO analysis is endopeptidase inhibitor activity. Label-free protein quantification showed no significant difference between glaucoma and cataract aqueous humor proteomes. At the same time, we found decrease in the level of apolipoprotein D as a marker of the pseudoexfoliation syndrome. The data are available from ProteomeXchange repository (PXD002623).
Institute of Biomedical Chemistry, Moscow, Russia.
Full articleClassification:
3.12 Proteomics (Part of: 3 Laboratory methods)
9.4.4.1 Exfoliation syndrome (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders > 9.4.4 Glaucomas associated with disorders of the lens)
2.6.3 Compostion (Part of: 2 Anatomical structures in glaucoma > 2.6 Aqueous humor dynamics)
