advertisement

Topcon

Abstract #69024 Published in IGR 18-1

Automated Axon Counting in Rodent Optic Nerve Sections with AxonJ

Zarei K; Scheetz TE; Christopher M; Miller K; Hedberg-Buenz A; Tandon A; Anderson MG; Fingert JH; Abràmoff MD
Scientific reports 2016; 6: 26559


We have developed a publicly available tool, AxonJ, which quantifies the axons in optic nerve sections of rodents stained with paraphenylenediamine (PPD). In this study, we compare AxonJ's performance to human experts on 100x and 40x images of optic nerve sections obtained from multiple strains of mice, including mice with defects relevant to glaucoma. AxonJ produced reliable axon counts with high sensitivity of 0.959 and high precision of 0.907, high repeatability of 0.95 when compared to a gold-standard of manual assessments and high correlation of 0.882 to the glaucoma damage staging of a previously published dataset. AxonJ allows analyses that are quantitative, consistent, fully-automated, parameter-free, and rapid on whole optic nerve sections at 40x. As a freely available ImageJ plugin that requires no highly specialized equipment to utilize, AxonJ represents a powerful new community resource augmenting studies of the optic nerve using mice.

Full article

Classification:

5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
2.15 Optic nerve (Part of: 2 Anatomical structures in glaucoma)



Issue 18-1

Change Issue


advertisement

Oculus