advertisement
Abstract #69106 Published in IGR 18-1
Transplantation of iPSC-derived TM cells rescues glaucoma phenotypes in vivo
Zhu W; Gramlich OW; Laboissonniere L; Jain A; Sheffield VC; Trimarchi JM; Tucker BA; Kuehn MHProceedings of the National Academy of Sciences of the United States of America 2016; 113: E3492-500
Glaucoma is a common cause of vision loss or blindness and reduction of intraocular pressure (IOP) has been proven beneficial in a large fraction of glaucoma patients. The IOP is maintained by the trabecular meshwork (TM) and the elevation of IOP in open-angle glaucoma is associated with dysfunction and loss of the postmitotic cells residing within this tissue. To determine if IOP control can be maintained by replacing lost TM cells, we transplanted TM-like cells derived from induced pluripotent stem cells into the anterior chamber of a transgenic mouse model of glaucoma. Transplantation led to significantly reduced IOP and improved aqueous humor outflow facility, which was sustained for at least 9 wk. The ability to maintain normal IOP engendered survival of retinal ganglion cells, whose loss is ultimately the cause for reduced vision in glaucoma. In vivo and in vitro analyses demonstrated higher TM cellularity in treated mice compared with littermate controls and indicated that this increase is primarily because of a proliferative response of endogenous TM cells. Thus, our study provides in vivo demonstration that regeneration of the glaucomatous TM is possible and points toward novel approaches in the treatment of this disease.
Department of Ophthalmology and Visual Sciences, University of Iowa, Iowa City, IA 52242; Center for the Prevention and Treatment of Visual Loss, Iowa City Veterans Affairs Medical Center, Iowa City, IA 52242;
Full articleClassification:
2.17 Stem cells (Part of: 2 Anatomical structures in glaucoma)
2.5.1 Trabecular meshwork (Part of: 2 Anatomical structures in glaucoma > 2.5 Meshwork)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
