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Abstract #75409 Published in IGR 19-2
Biolistic Labeling of Retinal Ganglion Cells
Santina LD; Ou YMethods in molecular biology (Clifton, N.J.) 2018; 1695: 161-170
Labeling of cellular structures is of fundamental importance in the investigation of diseases of the central nervous system. Biolistic labeling of retinal ganglion cells (RGCs) allows visualization of dendritic and synaptic structures of RGCs in retinal explants from animal models of experimental glaucoma. This technique sparsely labels RGCs, and, due to the stochastic nature of the particle delivery, all RGC types can be potentially observed in the labeled tissue. Quantification of dendritic and synaptic properties permits examination of the specific alterations to RGC morphology at different stages of degeneration, such as dendritic shrinkage and excitatory synapse loss.
Department of Ophthalmology, University of California, San Francisco, 10 Koret Way, San Francisco, CA, 94143, USA. luca.dellasantina@ucsf.edu.
Full articleClassification:
3.13.3 RGC Imaging (Part of: 3 Laboratory methods > 3.13 In vivo imaging)