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Miscellaneous (17)

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Abstract #75410 Published in IGR 19-2

Anterograde Tract Tracing for Assaying Axonopathy and Transport Deficits in Glaucoma

Crish SD; Schofield BR
Methods in molecular biology (Clifton, N.J.) 2018; 1695: 171-185

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Whether to stage degeneration or investigate early pathology in glaucoma, examination of axonal structure and function is essential. There are a wide variety of methods available to investigators using animal models of glaucoma, with varying utilities depending on the questions asked. Here, we describe the use of anterograde neuronal tract tracing using cholera toxin B (CTB) for the determination of axon transport integrity of the retinofugal projection. This method reveals the structure of the retinal axons as well as the functional integrity of anterograde transport systems.

Department of Pharmaceutical Sciences, Northeast Ohio Medical University, RGE-135, 4209 St. Rt. 44, Rootstown, OH, 44272, USA. scrish@neomed.edu.

Full article

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Classification:

3.13.3 RGC Imaging (Part of: 3 Laboratory methods > 3.13 In vivo imaging)

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