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Abstract #75412 Published in IGR 19-2
3D Histomorphometric Reconstruction and Quantification of the Optic Nerve Head Connective Tissues
Yang H; Reynaud J; Lockwood H; Williams G; Hardin C; Reyes L; Gardiner SK; Burgoyne CFMethods in molecular biology (Clifton, N.J.) 2018; 1695: 207-267
Accurately characterizing the 3D geometry of the optic nerve head neural and connective tissues has been the goal of a large and important body of scientific work. In the present report, we summarize our methods for the high-resolution, digital, 3D histomorphometric reconstruction of the optic nerve head tissues, including their visualization, parameterization, and quantification. In addition, we present our methods for between-eye comparisons of this anatomy, and their use to determine animal-specific and experiment-wide experimental glaucoma versus Control eye differences in the unilateral, monkey experimental glaucoma model. Finally, we demonstrate its application to finite element modeling, 3D optic nerve head reconstruction of other species, and 3D optic nerve head reconstructions using other imaging modalities.
Full article
Classification:
2.14 Optic disc (Part of: 2 Anatomical structures in glaucoma)
3.1 Microscopy (Part of: 3 Laboratory methods)
3.3 Immunohistochemistry (Part of: 3 Laboratory methods)