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Miscellaneous (41)

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Abstract #77179 Published in IGR 19-3

TGF-β2 induces proliferation and inhibits apoptosis of human Tenon capsule fibroblast by miR-26 and its targeting of CTGF

Bao H; Jiang K; Meng K; Liu W; Liu P; Du Y; Wang D
Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie 2018; 104: 558-565

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AIM: The aim of this study was to research the effect of TGF-β2 on human enon capsule fibroblasts proliferation and apoptosis and its potential mechanism. METHODS: Human eyeball fascia tissues (n = 45) were derived from ocular fascia tissues of patients who were underwent glaucoma filtration surgery, and Tenon capsule fibroblasts were obtained from these tissues. Liposome-mediated transfection, CCK8 assay, Hoechst33258 staining, qRT-PCR detection, western blot, and luciferase reporter assay were performed. RESULTS: TGF-β2 promoted proliferation and inhibited apoptosis of human Tenon capsule fibroblasts in a dose-dependent manner. TGF-β2 induced down-regulation of miR-26 and up-regulation of CTGF in a dose-dependent manner. CTGF was the target gene of miR-26 and miR-26 had a negative regulatory effect on CTGF expression. miR-26 up-regulation could significantly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). Down-regulation of CTGF could markly decrease proliferation and increase apoptosis of human Tenon capsule fibroblasts after induced by TGF-β2 (P < 0.05). CONCLUSION: miR-26 could inhibit proliferation and promote apoptosis of human Tenon capsule fibroblasts after they were induced by TGF-β2 through suppressing CTGF expression.

Department of Ophthalmology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China; Department of Ophthalmology, Central Hospital of Taian, Taian, Shandong, 271000, China.

Full article

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Classification:

12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
3.6 Cellular biology (Part of: 3 Laboratory methods)
3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
3.9 Pathophysiology (Part of: 3 Laboratory methods)

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