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Abstract #79090 Published in IGR 20-1
Cyanin Chloride Inhibits Hyperbaric Pressure-Induced Decrease of Intracellular Glutamate-Aspartate Transporter in Rat Retinal Müller Cells
Chen X; Wang Y; Han F; Ke MJournal of Ophthalmology 2018; 2018: 6128470
PURPOSE: Glaucoma is the leading cause of irreversible blindness throughout the world. The pathogenesis of glaucoma is complex, and neuroprotection is a crucial aspect of therapy. High concentrations of extracellular glutamate are toxic to the optic nerve. The glutamate-aspartate transporter (GLAST) in retinal Müller cells is involved in the development of glaucoma. Anthocyanin has been reported to protect retinal neurons. We hypothesize that cyanin chloride, a type of anthocyanin, can inhibit hyperbaric pressure-induced GLAST decreases in cultured rat retinal Müller cells and may serve as a potential neuroprotective agent in glaucoma treatment. MATERIALS AND METHODS: Sprague Dawley rat Müller cells were cultured in a hyperbaric pressure device at 60 mmHg additional pressure and treated with cyanin chloride (10 mol/L, 30 mol/L, or 50 mol/L) or vehicle for 2 hours. Cell survival rates (SRs) were evaluated by an MTT assay. GLAST mRNA and protein expression were determined by western blot and RT-PCR analyses, respectively. RESULTS: Cell SR was significantly decreased in the 60 mmHg additional hyperbaric pressure group compared to the control group ( < 0.01). Cyanin chloride treatment significantly improved SR under 60 mmHg additional pressure ( < 0.01). GLAST mRNA and protein expression levels in Müller cells were significantly reduced in the 60 mmHg hyperbaric pressure group compared to the control group ( < 0.01), but cyanin chloride significantly inhibited hyperbaric pressure-induced decreases in GLAST expression ( < 0.01). CONCLUSION: Our results support our hypothesis and demonstrate that cyanin chloride can protect rat retinal Müller cells from hyperbaric pressure-induced decreases of GLAST.
Department of Ophthalmology, Zhongnan Hospital, Wuhan University, Wuhan, China.
Full articleClassification:
3.8 Pharmacology (Part of: 3 Laboratory methods)
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
11.8 Neuroprotection (Part of: 11 Medical treatment)
