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1 General aspects (0)   1.1 Epidemiology (9)   1.2 Population genetics (2)   1.3 Pathogenesis (3)   1.4 Quality of life (16)   1.5 Glaucomas as cause of blindness (2)   1.6 Prevention and screening (11) 2 Anatomical structures in glaucoma (0)   2.1 Conjunctiva (5)   2.2 Cornea (13)   2.3 Sclera (5)   2.4 Anterior chamber angle (1)   2.5 Meshwork (0)     2.5.1 Trabecular meshwork (8)     2.5.2 Schlemms canal (2)     2.5.3 Scleral outlet channels (0)   2.6 Aqueous humor dynamics (0)     2.6.1 Production (0)     2.6.2 Outflow (1)       2.6.2.1 Trabecular meshwork (0)       2.6.2.2 Uveoscleral (0)     2.6.3 Compostion (3)   2.7 Episcleral veins and venous pressure (3)   2.8 Iris (4)   2.9 Ciliary body (0)   2.10 Lens (2)   2.11 Vitreous body (2)   2.12 Choroid, peripapillary choroid, peripapillary atrophy (10)   2.13 Retina and retinal nerve fibre layer (23)   2.14 Optic disc (31)   2.15 Optic nerve (5)   2.16 Chiasma and retrochiasmal central nervous system (4)   2.17 Stem cells (2)   2.20 Other (0) 3 Laboratory methods (0)   3.1 Microscopy (0)   3.2 Electron microscopy (0)   3.3 Immunohistochemistry (0)   3.4 Molecular genetics (0)     3.4.1 Linkage studies (0)     3.4.2 Gene studies (16)   3.5 Molecular biology incl. 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Abstract #79826 Published in IGR 20-2

TGF-β1 Induces Human Tenon's Fibroblasts Fibrosis via miR-200b and Its Suppression of PTEN Signaling

Tong J; Chen F; Du W; Zhu J; Xie Z
Current Eye Research 2019; 44: 360-367

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BACKGROUND: To determine the involvement of MicroRNA-200b (miR-200b) in human Tenon's fibroblasts (HTFs) fibrosis. METHODS: HTFs were treated with 10 ng/ml TGF-β1 for 24 h. Expression of α-smooth muscle actin (α-SMA), Fibronectin and Collagen1A1 (COL1A1) was assessed by western blot and immunofluorescence. MiR-200b was detected by quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), and its potential target genes were validated by luciferase assay. The effects of miR-200b and PTEN (the target gene) on HTFs fibrogenesis were evaluated using miR-200b mimics or inhibitor transfected HTFs, and confirmed with PTEN-specific small interfering RNA (siRNA) and PTEN inhibitor. RESULTS: TGF-β1 up-regulated miR-200b and promoted the expression of α-SMA, Fibronectin and COL1A1 in HTFs, and the effects could be strengthened or hampered by adding miR-200b mimics or inhibitor, respectively. Luciferase assay identified PTEN as the target gene of miR-200b, and decreased PTEN level due to up-regulation of miR-200b was found to correlate with intensified expression of α-SMA, Fibronectin and COL1A1. Determination of PTEN's role in fibrosis was carried out by adding PTEN specific siRNA or PTEN inhibitor into HTFs, which led to intensified expression of fibrosis-related proteins. CONCLUSION: TGF-β1-mediated miR-200b induces HTFs fibrosis through suppressing PTEN signaling pathway.

a Department of Ophthalmology, Clinical Medical College , Yangzhou University , Yangzhou , Jiangsu Province , China.

Full article

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Classification:

3.5 Molecular biology incl. SiRNA (Part of: 3 Laboratory methods)
3.6 Cellular biology (Part of: 3 Laboratory methods)
12.8.2 With tube implant or other drainage devices (Part of: 12 Surgical treatment > 12.8 Filtering surgery)

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