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Abstract #8035 Published in IGR 4-3
Modifying subscleral fluid pressure in an experimental model of mitomycin-C diffusion
Georgopoulos M; Vass C; Vatanparast ZExperimental Eye Research 2002; 75: 677-681
BACKGROUND: Episcleral application of mitomycin-C (MMC) during glaucoma filtration surgery hinders the postoperative wound healing. Diffusion through the sclera might result in a toxic effect on the ciliary body resulting in reduced aqueous humor production, leading to postoperative hypotony. The authors developed an experimental model to investigate the influence of intraocular pressure (IOP) on the diffusion of MMC through the sclera and in subscleral compartments. METHODS: Scleral quadrants of ten human donor eyes were mounted on PMMA tubes filled with saline imitating the intraocular volume. By height variation of a coupled infusion line different IOPs were simulated (0, 8, 23 and 80 mmHg). Additionally the model included a subscleral sponge to mimic the compartment of the ciliary body. The episcleral sides of the scleral quadrants were exposed for one minute to sponges soaked with 200 g ml-1 MMC. An 8-mm-diameter scleral disc was punched out with a trephine and horizontally dissected with a kryotome. The MMC concentrations of scleral layers, epi- and subscleral sponges and the fluid within the tubes were analyzed by means of high-performance liquid chromatography. RESULTS: The MMC concentration gradually declined from the episcleral sponge (165 g ml-1) to the superficial (3.3 g ml-1) and deep scleral layers (1.2 g ml-1), and to the subscleral sponge (0.2 g ml-1). The authors were able to detect very small concentrations of MMC in the fluid within the PMMA tubes (0.01 g ml-1). CONCLUSIONS: The authors developed a new experimental in vitro model for investigating transscleral MMC diffusion. The different simulated IOPs had no effect on the concentration gradient through the investigated compartments of their model.
M. Georgopoulos, MD, Universitätsklinik für Augenheilkunde und Optometrie, Allgemeines Krankenhaus Wien, Währinger Gürtel 18-20, A-1090 Vienna, Austria. michael.georgopoulos@akh-wien.ac.at
Classification:
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
