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Abstract #81441 Published in IGR 20-3
Increased Plasma Levels of 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in Patients with Pseudoexfoliation Glaucoma
Kondkar AA; Sultan T; Azad TA; Tabussum L; Osman EA; Al-Obeidan SAJournal of Ophthalmology 2019; 2019: 8319563
PURPOSE: To investigate systemic oxidative stress-induced DNA damage in patients with pseudoexfoliation glaucoma (PXG), we estimated plasma levels of 8-hydroxy-2'-deoxyguanosine (8-OHdG) as a marker for oxidative DNA damage in comparison to controls. In addition, we also examined a combined effect of lysyl oxidase-like 1 () polymorphism status and 8-OHdG levels on PXG risk. MATERIALS AND METHODS: A retrospective case-control study was performed to estimate plasma levels of 8-OHdG in 41 PXG patients and 45 nonglaucomatous controls using the enzyme-linked immunosorbent assay (ELISA). The assay was performed in duplicate on an automated ELISA analyzer. Two common polymorphisms (rs1048661 and rs3835942) in gene were genotyped by Sanger sequencing. RESULTS: The mean and median levels of 8-OHdG were significantly increased in the PXG cases (=0.032) and male subjects (=0.041). Subjects with levels greater than the third quartile (75% percentile) exhibited a significant increased risk of PXG (odds ratio = 4.06, 95% confidence interval (CI = 1.11-14.80, =0.029)). Within- and between-group comparisons showed that the mean levels were higher in individuals carrying the risk variant (G/G), but not statistically significant. In logistic regression analysis, both 8-OHdG (=0.044) and rs3835942 (=0.012) showed a statistically significant effect on the PXG outcome. However, the effect was lost when age, sex, and rs1048661 were included. A significant positive correlation was observed between 8-OHdG levels and intraocular pressure (=0.284, =0.008) and cup/disc ratio (=0.233, =0.031). Furthermore, in receiver operating characteristic analysis, the area under the curve was statistically significant (=0.032) with a value of 0.635 (95% CI = 0.518-0.751). CONCLUSION: The study demonstrates an association of increased plasma levels of 8-OHdG in patients with PXG, supporting the role of oxidative stress, and increased oxidative DNA damage in PXG development.
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Classification:
9.4.4.1 Exfoliation syndrome (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders > 9.4.4 Glaucomas associated with disorders of the lens)
3.7 Biochemistry (Part of: 3 Laboratory methods)
9.4.15 Glaucoma in relation to systemic disease (Part of: 9 Clinical forms of glaucomas > 9.4 Glaucomas associated with other ocular and systemic disorders)
