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Abstract #8188 Published in IGR 4-3
Effects of elevated pressure on prostanoid receptor gene expression levels in human trabecular meshwork
Schneemann A; Hoyng PFJ; Kamphuis WOphthalmic Research 2002; 34: 314-318
The purpose of this study was to assess the effects of increased intraocular pressure (IOP) on the expression levels of the prostanoid receptor genes (DP, EP1-4, FP, IP, TP) in the trabecular meshwork of human donor eyes. Anterior segments of human donor eyes were perfused in an ex vivo anterior segment perfusion system under different pressure regimes. The expression levels of the prostanoid receptors and of several housekeeping genes were assessed by non-competitive real-time quantitative polymerase chain reaction (Q-PCR). Perfusion of the anterior segments for 24 hours at 10 mmHg, followed by 24 hours at 30 mmHg, caused a significant decrease in the expression of the EP2 receptor compared to a constant perfusion under 10 mmHg. No significant changes were found for the other prostanoid receptor transcripts. When the pressure was raised to 30 mmHg for only one or three hours, no changes in the EP2 receptor expression levels were evident. However, a transient DP and TP receptor expression increase was found after one hour of perfusion at 30 mmHg. Whether these changes in expression are part of a response of the trabecular meshwork cells in order to normalize IOP needs to be studied further.
A. Schneemann, MD, Netherlands Ophthalmic Research Institute, Meibergdreef 47, NL-1105 BA, Amsterdam, The Netherlands. a.schneemann@ioi.knaw.nl
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
3 Laboratory methods
