advertisement
Abstract #8194 Published in IGR 4-3
cDNA microarray analysis of gene expression changes induced by dexamethasone in cultured human trabecular meshwork cells
Ishibashi T; Takagi Y; Mori K; Naruse S; Nishino H; Yue BYJT; Kinoshita SInvestigative Ophthalmology and Visual Science 2002; 43: 3691-3697
PURPOSE: To profile gene expression changes induced by dexamethasone in cultured human trabecular meshwork (TM) cells and identify genes related to the occurrence of steroid-induced glaucoma. METHODS: At confluence, dexamethasone (final concentration 10-7 M in 0.1% ethanol) or vehicle alone (control, 0.1% ethanol) was applied to cultured human TM cells from eyes of four normal subjects. After seven days of application, a labelled cDNA probe was synthesized from extracted total RNA and hybridized to a human cDNA microarray containing 2400 genes. After hybridization, the tyramide signal was amplified, and the fluorescent signals on each microarray were scanned and analyzed. RESULTS: In dexamethasone-treated TM cells, simultaneous analysis of 2400 human genes indicated a more than two-fold increase in 30 genes. Five of these, myocilin (MYOC), decorin, insulin-like growth factor binding protein 2, ferritin L chain, and fibulin-1C, were the most upregulated genes with higher-than-control expression levels in all four experiments. Their upregulation was further confirmed by semiquantitative RT-PCR. Downregulation, with fluorescent signals decreased to less than a half, was found in 34 genes. The dexamethasone-induced expression changes in most of these TM cell genes have not been reported previously. CONCLUSIONS: cDNA microarray is a useful tool for gene expression analysis that confirms previous reports of upregulated mRNA expression of MYOC after treatment with dexamethasone in human TM cells. Changes in other genes subsequent to the treatment with dexamethasone may also reduce outflow facility, providing new insights into the pathogenesis of steroid-induced glaucoma.
T. Ishibashi, MD, Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
6 Clinical examination methods
