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Abstract #8198 Published in IGR 4-3
A feasible tool to detect mRNA expression of matrix metalloproteinases and their tissue inhibitors in human tenon's capsule
Huang YL; Liu CJ; Chiu AW; Wang YC; Huan SK; Lee FL; Chen SJ; Hsu WM; Hsieh SLOphthalmic Research 2002; 34: 375-379
PURPOSE: To determine the mRNA expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human specimens of Tenon's capsule. METHODS: Reverse transcription-polymerase chain reaction (RT-PCR) with specific primers for MMP-1, MMP-2, MMP-9, TIMP-1 and TIMP-2 was performed on tissue specimens obtained from patients with cataract, rhegmatogenous retinal detachment, glaucoma, or diabetes mellitus. RESULTS: Glyceraldehyde phosphate dehydrogenase (GAPDH) mRNA transcripts were detected in 26 (76.5%) of 34 specimens, with almost the same amount of expression in each of these samples. Messenger RNA expression of one or more of the MMPs/TIMPs could also be detected in all these 26 samples, but not in any sample without GAPDH expression. MMP-2, TIMP-1 and TIMP-2 were detected in 25 (96.2%) of the 26 samples with GAPDH expression, while MMP-1 and MMP-9 were detected with a lower percentage (34.6 and 19.2%, respectively). CONCLUSIONS: The feasibility of RT-PCR with GAPDH as an internal standard to determine mRNA transcripts of the MMPs and TIMPs in the subconjunctival Tenon's capsule was demonstrated.
C.J. Liu, MD, Department of Ophthalmology, Taipei Veterans General Hospital, No. 201, Shih-Pai Road, Sec. 2, Taipei, Taiwan. jlliu@vghtpe.gov.tw
Classification:
2.1 Conjunctiva (Part of: 2 Anatomical structures in glaucoma)
3 Laboratory methods