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Abstract #8326 Published in IGR 4-3
Effect of L-arginine and L-NAME on proliferation and apoptosis of cultured bovine trabecular meshwork cells
Xue W; Du S; Li YChinese Ophthalmic Research 2002; 20: 401-403
OBJECTIVE: To study the proliferation and apoptosis of cultured bovine trabecular meshwork (TM) cells influenced by L-arginine and L-NAME. METHODS: TM cells with L-arginine and L-NAME were incubated for 48 hours. In the control group, no medicine was added. In the treatment groups, concentrations of L-arginine and L-NAME were 1 x 10-7 mol/L, 1 x 10-6 mol/L, 1 x 10-5 mol/L, 1 x 10-4 mol/L, 1 x 10-3 Mol/L, 1 x 10-2 mol/L, respectively. NO2- in supernate, the proliferation and apoptosis of TM cells were detected by Griess assay, terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL), and MTT assay. RESULTS: ≥ 1 x 10-4 mol/L of L-arginine could induce the apoptosis of TM cells and inhibit the proliferation of TM cells by promoting the synthesis of NO. However, ≥ 1 x 10-5 mol/L of L-NAME could accelerate the proliferation of TM cells by inhibiting the production of NO. CONCLUSION: Nitric oxide in high concentrations can induce TM cell apoptosis and suppress cell proliferation. LA: Chinese
S. Du, MD, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
Classification:
2.5 Meshwork (Part of: 2 Anatomical structures in glaucoma)
4 Tissue culture of ocular cells
