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Abstract #84566 Published in IGR 21-1
Inhibitive effect of TAK-242 on Tenon's capsule fibroblasts proliferation in rat eyes
Liang L; Zhu MN; Chen BJ; Wang Z; He LY; Zhang RInternational Journal of Ophthalmology 2019; 12: 1699-1707
AIM: To study the inhibition effect of TAK-242 on the proliferation of rat eye Tenon's capsule fibroblasts the toll-like receptor 4 (TLR4) signaling pathway. METHODS: SD rat Tenon's capsule fibroblasts were extracted and cultured, then the cells were divided into normal control group, lipopolysaccharide (LPS) group (10 g/mL LPS) and TAK-242 group (1 µmol/L TAK-242, and 10 µg/mL LPS after 30min). The expressions of TLR4, transforming growth factor-β1 (TGF-β1) and interleukin-6 (IL-6) in each group were detected by Western blot and reverse transcriptase-polymerase chain reaction (RT-PCR). Cell proliferation was detected by cell counting kit-8 (CCK-8). RESULTS: Double immunofluorescent labeling in the extracted cells showed negative keratin staining and positive vimentin staining. Western blot showed that the LPS group had the highest expression of TLR4 and TGF-β1 (<0.01). Enzyme linked immunosorbent assay (ELISA) also showed that the secretion of IL-6 was the highest in LPS group (<0.01). But there was no significant difference in TLR4 and TGF-1, as well as IL-6 expressions between the TAK-242 group and the normal control group (>0.05). RT-PCR showed that the IL-6 mRNA expression in LPS group was the highest in the three groups (<0.01). CONCLUSION: TAK-242 inhibits the proliferation of LPS-induced Tenon's capsule fibroblasts and the release of inflammatory factors by regulating the TLR4 signaling pathway, providing a new idea for reducing the scarring of the filter passage after glaucoma filtration surgery.
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Classification:
5.1 Rodent (Part of: 5 Experimental glaucoma; animal models)
12.8.10 Woundhealing antifibrosis (Part of: 12 Surgical treatment > 12.8 Filtering surgery)
3.8 Pharmacology (Part of: 3 Laboratory methods)